The University of Queensland

Size exclusion chromatography: a simple and reliable method for exosome purification

Lobb, Richard and Moller, Andreas (2017). Size exclusion chromatography: a simple and reliable method for exosome purification. In Winston Patrick Kuo and Shidong Jia (Ed.), Extracellular vesicles: methods and protocols (pp. 105-110) New York, NY, United States: Humana Press. doi:10.1007/978-1-4939-7253-1_9

Analysis of secreted small extracellular vesicles from activated human microglial cell lines reveals distinct pro‐ and anti‐inflammatory proteomic profiles

Niu, Xueming, Zhang, Zhen, Zhou, Quan, Wuethrich, Alain, Lobb, Richard and Trau, Matt (2024). Analysis of secreted small extracellular vesicles from activated human microglial cell lines reveals distinct pro‐ and anti‐inflammatory proteomic profiles. Proteomics, 24 (11) 2300094, e2300094. doi: 10.1002/pmic.202300094

A mesoporous gold biosensor to investigate immune checkpoint protein heterogeneity in single lung cancer cells

Ahmed, Emtiaz, Masud, Mostafa Kamal, Komatineni, Prathyusha, Dey, Shuvashis, Lobb, Richard, Hossain, Md Shahriar A., Möller, Andreas, Yamauchi, Yusuke, Sina, Abu Ali Ibn and Trau, Matt (2024). A mesoporous gold biosensor to investigate immune checkpoint protein heterogeneity in single lung cancer cells. Biosensors and Bioelectronics, 249 115984, 115984. doi: 10.1016/j.bios.2023.115984

Glycan profiling in small extracellular vesicles with a SERS microfluidic biosensor identifies early malignant development in lung cancer

Zhou, Quan, Niu, Xueming, Zhang, Zhen, O'Byrne, Kenneth, Kulasinghe, Arutha, Fielding, David, Möller, Andreas, Wuethrich, Alain, Lobb, Richard J. and Trau, Matt (2024). Glycan profiling in small extracellular vesicles with a SERS microfluidic biosensor identifies early malignant development in lung cancer. Advanced Science, e2401818. doi: 10.1002/advs.202401818

Tracking the EMT-like phenotype switching during targeted therapy in melanoma by analyzing extracellular vesicle phenotypes

Zhou, Quan, Wang, Jing, Zhang, Zhen, Wuethrich, Alain, Lobb, Richard J. and Trau, Matt (2023). Tracking the EMT-like phenotype switching during targeted therapy in melanoma by analyzing extracellular vesicle phenotypes. Biosensors and Bioelectronics, 244 115819, 115819. doi: 10.1016/j.bios.2023.115819

A microfluidic liquid biopsy platform to monitor protein biomarker heterogeneity in single circulating therapy‐resistance cancer cell

Ahmed, Emtiaz, Shanmugasundaram, Karthik Balaji, Li, Junrong, Behren, Andreas, Lobb, Richard, Möller, Andreas, Wuethrich, Alain, Dey, Shuvashis, Sina, Abu Ali Ibn and Trau, Matt (2023). A microfluidic liquid biopsy platform to monitor protein biomarker heterogeneity in single circulating therapy‐resistance cancer cell. Advanced Sensor Research, 2 (12) ARTN 2300059. doi: 10.1002/adsr.202300059

A universal reagent for detection of emerging diseases using bioengineered multifunctional yeast nanofragments

Li, Junrong, Howard, Christopher B., Dey, Shuvashis, Lowry, Kym, Whiley, David M., Puttick, Simon, Rose, Stephen, Lobb, Richard J., Wuethrich, Alain, Edwardraja, Selvakumar and Trau, Matt (2023). A universal reagent for detection of emerging diseases using bioengineered multifunctional yeast nanofragments. Nature Nanotechnology, 18 (10), 1222-1229. doi: 10.1038/s41565-023-01415-1

An epithelial-to-mesenchymal transition induced extracellular vesicle prognostic signature in non-small cell lung cancer

Lobb, Richard J., Visan, Kekoolani S., Wu, Li-Ying, Norris, Emma L., Hastie, Marcus L., Everitt, Sarah, Yang, Ian A., Bowman, Rayleen V., Siva, Shankar, Larsen, Jill E., Gorman, Jeffrey J., MacManus, Michael, Leimgruber, Antoine, Fong, Kwun M. and Möller, Andreas (2023). An epithelial-to-mesenchymal transition induced extracellular vesicle prognostic signature in non-small cell lung cancer. Communications biology, 6 (1) 68, 68. doi: 10.1038/s42003-022-04350-4

Digital decoding of single extracellular vesicle phenotype differentiates early malignant and benign lung lesions

Li, Junrong, Sina, Abu A. I., Antaw, Fiach, Fielding, David, Möller, Andreas, Lobb, Richard, Wuethrich, Alain and Trau, Matt (2023). Digital decoding of single extracellular vesicle phenotype differentiates early malignant and benign lung lesions. Advanced Science, 10 (1) 2204207, e2204207. doi: 10.1002/advs.202204207

Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles

Visan, Kekoolani S., Lobb, Richard J., Ham, Sunyoung, Lima, Luize G., Palma, Carlos, Edna, Chai Pei Zhi, Wu, Li-Ying, Gowda, Harsha, Datta, Keshava K., Hartel, Gunter, Salomon, Carlos and Möller, Andreas (2022). Comparative analysis of tangential flow filtration and ultracentrifugation, both combined with subsequent size exclusion chromatography, for the isolation of small extracellular vesicles. Journal of Extracellular Vesicles, 11 (9) 12266, 12266. doi: 10.1002/jev2.12266

Blood-derived extracellular vesicle-associated miR-3182 detects non-small cell lung cancer patients

Visan, Kekoolani S., Lobb, Richard J., Wen, Shu Wen, Bedo, Justin, Lima, Luize G., Krumeich, Sophie, Palma, Carlos, Ferguson, Kaltin, Green, Ben, Niland, Colleen, Cloonan, Nicole, Simpson, Peter T., McCart Reed, Amy E., Everitt, Sarah J., MacManus, Michael P., Hartel, Gunter, Salomon, Carlos, Lakhani, Sunil R., Fielding, David and Möller, Andreas (2022). Blood-derived extracellular vesicle-associated miR-3182 detects non-small cell lung cancer patients. Cancers, 14 (1) 257, 257. doi: 10.3390/cancers14010257

Generation of nanoyeast single-chain variable fragments as high-avidity biomaterials for dengue virus detection

Farokhinejad, Fahimeh, Lane, Rebecca E., Lobb, Richard J., Edwardraja, Selvakumar, Wuethrich, Alain, Howard, Christopher B. and Trau, Matt (2021). Generation of nanoyeast single-chain variable fragments as high-avidity biomaterials for dengue virus detection. ACS Biomaterials Science and Engineering, 7 (12) acsbiomaterials.1c01001, 5850-5860. doi: 10.1021/acsbiomaterials.1c01001

Amplification-free SARS-CoV-2 detection using nanoyeast-scFv and ultrasensitive plasmonic nanobox-integrated nanomixing microassay

Li, Junrong, Wuethrich, Alain, Edwardraja, Selvakumar, Lobb, Richard J., Puttick, Simon, Rose, Stephen, Howard, Christopher B. and Trau, Matt (2021). Amplification-free SARS-CoV-2 detection using nanoyeast-scFv and ultrasensitive plasmonic nanobox-integrated nanomixing microassay. Analytical Chemistry, 93 (29) acs.analchem.1c01657, 10251-10260. doi: 10.1021/acs.analchem.1c01657

Characterizing the heterogeneity of small extracellular vesicle populations in multiple cancer types via an ultrasensitive chip

Wang, Jing, Wuethrich, Alain, Lobb, Richard J., Antaw, Fiach, Sina, Abu Ali Ibn, Lane, Rebecca E., Zhou, Quan, Zieschank, Chloe, Bell, Caroline, Bonazzi, Vanessa F., Aoude, Lauren G., Everitt, Sarah, Yeo, Belinda, Barbour, Andrew P., Möller, Andreas and Trau, Matt (2021). Characterizing the heterogeneity of small extracellular vesicle populations in multiple cancer types via an ultrasensitive chip. ACS Sensors, 6 (9) acssensors.1c00358, 3182-3194. doi: 10.1021/acssensors.1c00358

Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution

Lima, Luize G., Ham, Sunyoung, Shin, Hyunku, Chai, Edna P. Z., Lek, Erica S. H., Lobb, Richard J., Müller, Alexandra F., Mathivanan, Suresh, Yeo, Belinda, Choi, Yeonho, Parker, Belinda S. and Möller, Andreas (2021). Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution. Nature Communications, 12 (1) 3543, 1-12. doi: 10.1038/s41467-021-23946-8

The evolving translational potential of small extracellular vesicles in cancer

Möller, Andreas and Lobb, Richard J. (2020). The evolving translational potential of small extracellular vesicles in cancer. Nature Reviews Cancer, 20 (12), 697-709. doi: 10.1038/s41568-020-00299-w

The role of exosomes in the promotion of epithelial-to-mesenchymal transition and metastasis

Visan, Kekoolani S., Lobb, Richard J. and Moller, Andreas (2020). The role of exosomes in the promotion of epithelial-to-mesenchymal transition and metastasis. Frontiers in Bioscience - Landmark, 25 (6) 4846, 1022-1057. doi: 10.2741/4846

Breast cancer-derived exosomes reflect the cell-of-origin phenotype

Wen, Shu Wen, Lima, Luize G., Lobb, Richard J., Norris, Emma L., Hastie, Marcus L., Krumeich, Sophie and Möller, Andreas (2019). Breast cancer-derived exosomes reflect the cell-of-origin phenotype. Proteomics, 19 (8) 1800180, 1800180. doi: 10.1002/pmic.201800180

Secreted cellular prion protein binds doxorubicin and correlates with anthracycline resistance in breast cancer

Wiegmans, Adrian P., Saunus, Jodi M., Ham, Sunyoung, Lobb, Richard J., Kutasovic, Jamie R., Dalley, Andrew J., Miranda, Mariska, Atkinson, Caroline, Foliaki, Simote T., Ferguson, Kaltin, Niland, Colleen, Johnstone, Cameron N., Lewis, Victoria, Collins, Steven, Lakhani, Sunil R., Al-Ejeh, Fares and Möller, Andreas (2019). Secreted cellular prion protein binds doxorubicin and correlates with anthracycline resistance in breast cancer. JCI Insight, 5 (6) ARTN e124092. doi: 10.1172/jci.insight.124092

The interaction of selenium with chemotherapy and radiation on normal and malignant human mononuclear blood cells

Lobb, Richard J., Jacobson, Gregory M., Cursons, Ray T. and Jameson, Michael B. (2018). The interaction of selenium with chemotherapy and radiation on normal and malignant human mononuclear blood cells. International Journal of Molecular Sciences, 19 (10) 3167, 3167. doi: 10.3390/ijms19103167

Breast cancer-derived exosomes alter macrophage polarization via gp130/STAT3 signaling

Ham, Sunyoung, Lima, Luize G., Chai, Edna Pei Zhi, Muller, Alexandra, Lobb, Richard J., Krumeich, Sophie, Wen, Shu Wen, Wiegmans, Adrian P. and Moeller, Andreas (2018). Breast cancer-derived exosomes alter macrophage polarization via gp130/STAT3 signaling. Frontiers in Immunology, 9 (MAY) 871, 871. doi: 10.3389/fimmu.2018.00871

Oncogenic transformation of lung cells results in distinct exosome protein profile similar to the cell of origin

Lobb, Richard J., Hastie, Marcus L., Norris, Emma L., van Amerongen, Rosa, Gorman, Jeffrey J. and Möller, Andreas (2017). Oncogenic transformation of lung cells results in distinct exosome protein profile similar to the cell of origin. Proteomics, 17 (23-24) 1600432, 1-10. doi: 10.1002/pmic.201600432

Exosomes derived from mesenchymal non-small cell lung cancer cells promote chemoresistance

Lobb, Richard J., van Amerongen, Rosa, Wiegmans, Adrian, Ham, Sunyoung, Larsen, Jill E. and Moller, Andreas (2017). Exosomes derived from mesenchymal non-small cell lung cancer cells promote chemoresistance. International Journal of Cancer, 141 (3), 614-620. doi: 10.1002/ijc.30752

An electrochemical method for the detection of disease-apecific exosomes

Yadav, Sharda, Boriachek, Kseniia, Islam, Md Nazmul, Lobb, Richard, Moller, Andreas, Hill, Michelle M., Hossain, Md Shahriar Al, Nguyen, Nam-Trung and Shiddiky, Muhammad J. A. (2017). An electrochemical method for the detection of disease-apecific exosomes. ChemElectroChem, 4 (4), 967-971. doi: 10.1002/celc.201600391

Unique molecular profile of exosomes derived from primary human proximal tubular epithelial cells under diseased conditions

Wang, Xiangju, Wilkinson, Ray, Kildey, Katrina, Potriquet, Jeremy, Mulvenna, Jason, Lobb, Richard J., Moller, Andreas, Cloonan, Nicole, Mukhopadhyay, Pamela, Kassianos, Andrew J. and Healy, Helen (2017). Unique molecular profile of exosomes derived from primary human proximal tubular epithelial cells under diseased conditions. Journal of Extracellular Vesicles, 6 (1) 1314073, 1314073-1314073. doi: 10.1080/20013078.2017.1314073

Exosomes: key mediators of metastasis and pre-metastatic niche formation

Lobb, Richard J., Lima, Luize G. and Moller, Andreas (2017). Exosomes: key mediators of metastasis and pre-metastatic niche formation. Seminars in Cell and Developmental Biology, 67, 3-10. doi: 10.1016/j.semcdb.2017.01.004

The biodistribution and immune suppressive effects of breast cancer-derived exosomes

Wen, Shu Wen, Sceneay, Jaclyn, Lima, Luize Goncalves, Wong, Christina S. F., Becker, Melanie, Krumeich, Sophie, Lobb, Richard J., Castillo, Vanessa, Wong, Ke Ni, Ellis, Sarah, Parker, Belinda S. and Moller, Andreas (2016). The biodistribution and immune suppressive effects of breast cancer-derived exosomes. Cancer Research, 76 (23), 6816-6827. doi: 10.1158/0008-5472.CAN-16-0868

Radiotherapy for non-small cell lung cancer induces DNA damage response in both irradiated and out-of-field normal tissues

Siva, Shankar, Lobachevsky, Pavel, MacManus, Michael P., Kron, Tomas, Möller, Andreas, Lobb, Richard J., Ventura, Jessica, Best, Nickala, Smith, Jai, Ball, David and Martin, Olga A. (2016). Radiotherapy for non-small cell lung cancer induces DNA damage response in both irradiated and out-of-field normal tissues. Clinical Cancer Research, 22 (19), 4817-4826. doi: 10.1158/1078-0432.CCR-16-0138

Optimized exosome isolation protocol for cell culture supernatant and human plasma

Lobb, Richard J., Becker, Melanie, Wen, Shu Wen, Wong, Christina S. F., Wiegmans, Adrian P., Leimgruber, Antione and Moller, Andreas (2015). Optimized exosome isolation protocol for cell culture supernatant and human plasma. Journal of Extracellular Vesicles, 4 (1) 27031, 27031. doi: 10.3402/jev.v4.27031

Exosomes in cancer metastasis: Novel targets for diagnosis and therapy?

Wen, Shu Wen, Lobb, Richard and Moller, Andreas (2014). Exosomes in cancer metastasis: Novel targets for diagnosis and therapy?. Cancer Forum, 38 (2), 116-120.

Purification and Analysis of Exosomes Derived From Primary Human Proximal Epithelial Cells (Ptec)

Wang, X., Kassianos, A. J., Potriquet, J., Lobb, R., Wilkinson, R. and Healy, H. (2015). Purification and Analysis of Exosomes Derived From Primary Human Proximal Epithelial Cells (Ptec). 51st Annual Scientific Meeting of the Australian and New Zealand Society of Nephrology, Canberra, Australia, 7-9 September 2015. HOBOKEN: WILEY-BLACKWELL.

Exosomes in the disease progression of non-small cell lung cancer (NSCLC) patients. Exosomes as biomarkers in NSCLC

Lobb, Richard John (2018). Exosomes in the disease progression of non-small cell lung cancer (NSCLC) patients. Exosomes as biomarkers in NSCLC. PhD Thesis, School of Medicine, The University of Queensland. doi: 10.14264/uql.2018.236